Rapid Ornithine Decarboxylase Test for the Identification of Enterobacteriaceae
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چکیده
منابع مشابه
Rapid ornithine decarboxylase test for the identification of enterobacteriaceae.
Conventional methods for detecting ornithine decarboxylase activity require an extended period of incubation. However, with a few simple modifications, accurate results were obtained within a few hours rather than several days. The broth medium was modified, primarily by omitting glucose and by decreasing the pH to 5.5. A 1-ml amount of this broth was inoculated with one colony and then overlai...
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Ornithine decarboxylase (ODC; EC 4.1.1.17), the first and key enzyme in polyamine biosynthesis (Figure 1), turns over extremely rapidly and is one of the most highly regulated enzymes known [1-3]. It is induced by many kinds of growth stimuli, and its suppression by specific inhibitors or mutation inhibits cellular growth and transformation [4,5]. A recent study showed that overexpression of OD...
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A rapid biochemical method for the determination of ornithine and lysine decarboxylase (EC 4.1.1.18) activity has been developed for use in the routine clinical laboratory. It is based on the detection of the amine end product produced in response to the single key amino acid added to a synthetic medium. A modified ninhydrin reagent is used to detect the amine after a chloroform extraction. Thi...
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ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1972
ISSN: 0003-6919
DOI: 10.1128/am.23.4.710-713.1972